AUTOMATION
Pipeting robots
Hamilton STARlet 8-Canal:
Liquid handling and assay assembly; equipped with Easypick automated Colony picker for selection of microorganisms.
Hamilton STARlet 96-Canal:
amplification and inoculation and additionally equipped with a unique Pin Tool for inoculation of up to 1536 colonies on solid medium
High Throughput growth and enzyme assays with incubation:
Cytomat 2c 450, 42 standard microtiter plates (4°C – 50°C)
Hamilton Rackrunner for plate transport between machines
2 Plate readers:
BioTek Synergy H1 with monochromator UV-Vis absorbance and luminescence detection and injection module
CHEMICAL CHARACTERIZATION
Flash chromatography puriFlash 450 system for bulk purifications
HPLC systems:
Agilent 1200, Diode Array Detector (DAD) and fraction collector (fraction volume 300 ul to 5mL)
Preparative HPLC system: Agilent 1260, Infinity Diode Array Detector (DAD) and fraction collector (fraction volumes either 14ml or 50mL)
Powerful and universal detector:
Corona Charged Aerosol Detector Veo RS CAD can be coupled to the analytical and preparative HPLC system.
Access to LC-MS Orbitrap (iBAM, IFA) and 400MHz NMR (DCH, BOKU)
STRAIN COLLECTION
The sequencing of microbial genomes predicts a much higher variety of microbial metabolites than previously assumed. This has recently lead researchers to focus on the potential exploitation of microbial genetic resources. Therefore, we have built and are continuously expanding our strain collection of fungi and bacteria. Most samples come from soil with also a significant collection of Candida species obtained from Austrian sources. Our approach searches and characterizes previously unknown bioactive compounds and enzymes from bacteria, fungi and other microorganisms. By mimicking natural conditions, biotic interactions between different organisms in different chemical and physical environments are possible. This promotes the production of specific metabolites.
ADDITIONAL EQUIPMENT
High throughput cell lysis (MP96), various fermentation Chambers, Incubators (also CO2), 2 clean benches, 5 bench places, 4 fume hoods, -80 freezer, CLSM and other Microscopes, Rotavapor and more. The Labs are BSL2 certified.
If you are interested and would like more information – just get in contact with us: office@bimm-research.at
